PURIFICATION OF A GLUCOSE MANNOSE-SPECIFIC LECTIN, ISOFORM-1, FROM SEEDS OF CRATYLIA-MOLLIS MART (CAMARATU BEAN)

A quantitatively main molecular form of Cratylia mollis lectin, isofor m 1 (iso 1) was purified by affinity chromatography on Sephadex G-75, followed by ion exchange chromatography on CM-cellulose. Another lecti n form was identified in the latter step. Iso 1 is specific for glucos e/mannose, with a main subunit of 31 kDa mol wt; the native protein is basic (pI 8.5-8.6) and the constituent polypeptides had a pi range of 5.15-7.75. An antibody to the protein was raised in a rabbit, and the conjugate was active in an immunosorbent assay.