PHOTOCHEMICALLY INDUCED FLUORESCENCE INVESTIGATION OF A BETA-CYCLODEXTRIN-AZURE-A INCLUSION COMPLEX AND DETERMINATION OF ANALYTICAL PARAMETERS

The photooxidation of Azure A and fluorescence properties of Azure A a nd its photoproduct have been investigated in aqueous media and in the presence of beta-cyclodextrin (beta-CD). The fluorescence intensity o f the complex formed between the photoproduct and beta-CD was found to be three times higher than that of the uncomplexed Azure A photoprodu ct. A complex formation constant of 110 +/- 40 M(-1) was calculated us ing the Benesi-Hildebrand treatment of the fluorescence emission data. Although the stoichiometry of the Azure A photoproduct: beta-CD compl ex was found to be 1:1, it seems that the Azure A structure is only pa rtially included. Calibration graphs were plotted for the free Azure A photoproduct and the photogenerated product included in beta-CD. The analytical parameters and quantification limits were determined.