9-ALPHA-HYDROXYLATION OF 4-ANDROSTENE-3,17-DIONE BY RESTING RHODOCOCCUS SP CELLS

Authors
ANGELOVA B MUTAFOV S AVRAMOVA T DIMOVA I BOYADJIEVA L
Citation
B. Angelova et al., 9-ALPHA-HYDROXYLATION OF 4-ANDROSTENE-3,17-DIONE BY RESTING RHODOCOCCUS SP CELLS, Process biochemistry, 31(2), 1996, pp. 179-184
Citations number
18
Categorie Soggetti
Biothechnology & Applied Migrobiology",Biology
Journal title
Process biochemistry → ACNP
ISSN journal
13595113
Volume
31
Issue
2
Year of publication
1996
Pages
179 - 184
Database
ISI
SICI code
1359-5113(1996)31:2<179:9O4BRR>2.0.ZU;2-4
Abstract
The effect of nitrogen and carbon nutrition, growth and biomass storag e on the effectiveness of microbial 9 alpha-hydroxylation of 4-androst ene-3,17-dione was studied. The transformation was carried out by rest ing Rhodococcus sp. cells in nutrient deficient tranformation medium a t different pHs as well as in the presence of several solvents of the steroid compounds. A conversion ratio of ca 0.75 was observed with sta tionary phase cells grown on medium containing glucose and non-dehydra ted casein hydrolysate. A strong influence of the nitrogen source on t he effectiveness of the process was noticed. The presence of different alcohols in the reaction medium was found to increase the substrate c onversion ratio to ca 0.85. A decrease in the transformation ability o f stored cells, was a logarithmic function of the storage time.