SEQUENCE AND EXPRESSION ANALYSIS OF THE HRPB PATHOGENICITY OPERON OF XANTHOMONAS-CAMPESTRIS PV VESICATORIA WHICH ENCODES 8 PROTEINS WITH SIMILARITY TO COMPONENTS OF THE HRP, YSC, SPA, AND FLI SECRETION SYSTEMS

Citation
S. Fenselau et U. Bonas, SEQUENCE AND EXPRESSION ANALYSIS OF THE HRPB PATHOGENICITY OPERON OF XANTHOMONAS-CAMPESTRIS PV VESICATORIA WHICH ENCODES 8 PROTEINS WITH SIMILARITY TO COMPONENTS OF THE HRP, YSC, SPA, AND FLI SECRETION SYSTEMS, Molecular plant-microbe interactions, 8(6), 1995, pp. 845-854
Citations number
69
Categorie Soggetti
Plant Sciences","Biothechnology & Applied Migrobiology",Biology
ISSN journal
08940282
Volume
8
Issue
6
Year of publication
1995
Pages
845 - 854
Database
ISI
SICI code
0894-0282(1995)8:6<845:SAEAOT>2.0.ZU;2-8
Abstract
In this paper we describe the molecular characterization of hrpB, the largest operon in the Xanthomonas campestris pv. vesicatoria hrp clust er. The hrpB region encompasses 6 kb and encodes eight putative protei ns, seven of which were expressed in Escherichia coli. The HrpB3 prote in is the only one carrying a signal peptide sequence at the N-terminu s and is a putative lipoprotein localized in the outer membrane of X. campestris pv. vesicatoria. The HrpB4 and HrpB8 proteins contain one a nd five putative transmembrane domains, respectively, and are most lik ely associated with the inner membrane. The HrpB3, HrpB5, HrpB6, and H rpB8 proteins show sequence similarity to putative components of diffe rent type III protein secretion pathways in bacteria. Examples include Hrp proteins from other plant pathogens, YscJ, YscN, YscL, and YscT o f Yersinia spp., and MxiJ, Spa47, and Spa29 of Shigella flexneri. The transcription start site and the hrpB promoter was identified. The min imal hrpB promoter region of 90 bp contains a novel sequence motif, th e PIP-box, which might play a role in transcription activation of the hrpB operon and possibly other plant-induced genes of X. campestris pv . vesicatoria.