THE IN-VITRO ACTIVITY OF TROSPECTOMYCIN AGAINST ANAEROBIC-BACTERIA

The authors evaluated the activity of trospectomycin, a new aminocycli tol which is characterized by good antibacterial and broad spectrum ac tivity, in comparison with clindamycin and ampicillin on a sample of r ecent isolates: Bacteroides fragilis (15 strains), Bacteroides urealyt icus (5 strains), Bacteroides vulgatus (5 strains), Bacteroides spp. ( 15 strains), Prevotella melaninogenica (6 strains), Porphyromonas asac charolytica (7 strains), Mobiluncus spp. (3 strains), Peptococcus nige r (3 strains), Peptococcus variabilis (3 strains), Peptococcus spp (30 strains), Peptostreptococcus anaerobius (5 strains), Peptostreptococc us asaccharolyticus (3 strains), Peptostreptococcus spp. (25 strains) and Propionibacterium spp. (7 strains). The minimum inhibitory concent ration (MIC) and minimum bactericidal concentration (MBC) were determi ned for all strains by microtiter serial dilutions in Wilkins-Chalgren broth in an anaerobic chamber in an atmosphere of 10% H-2, 10% CO2, 8 0% N-2. All the drugs tested exert their activity against Gram-positiv e and Gram-negative anaerobic isolates. In particular, trospectomycin is quite active against Grampositive cocci (MIC 90 = 4 - 8 mg/l), Gram -negative rods (MIC 90 = 8 - 16 mg/l), Gram-positive rods (MIC 90 = 4 mg/l) and Mobiluncus spp. (MIC 30 = 0.5 mg/l).