A. Tiveljung et al., BROAD-RANGE PCR AMPLIFICATION AND DNA-SEQUENCE ANALYSIS REVEALS VARIABLE MOTIFS IN 16S RIBOSOMAL-RNA GENES OF MOBILUNCUS SPECIES, APMIS. Acta pathologica, microbiologica et immunologica Scandinavica, 103(10), 1995, pp. 755-763
Using DNA primers based on highly conserved regions of bacterial 16S r
ibosomal RNA genes, a technique was established for detection of Mobil
uncus species by polymerase chain reaction (PCR) and hybridization ana
lysis. Part of the 16S rRNA genes of Mobiluncus mulieris, Mobliuncus c
urtisii and uncharacterized Mobiluncus strains were analyzed by broad-
range PCR amplification and direct DNA sequencing analysis. Sequence c
omparison of the partial 16S rRNA genes of Mobiluncus curtisii, Mobilu
ncus mulieris and atypical Mobiluncus strains studied indicated genus
and species-specific motifs within the variable regions V3, V4 and V9
of 16S ribosomal DNAs. A Mobiluncus curtisii-specific primer, located
within the variable region V3 of the 16S rRNA gene, was designed for S
outhern blot hybridization analysis of broad-range PCR products. Broad
-range amplification combined with a M. curtisii-specific hybridizatio
n probe, Mob V3, distinguished between Mobiluncus curtisii, Mobiluncus
mulieris, and atypical Mobiluncus strains.