NOVEL GUS EXPRESSION PATTERNS FOLLOWING TRANSPOSITION OF AN ENHANCER TRAP DS ELEMENT IN ARABIDOPSIS

Citation
Vi. Klimyuk et al., NOVEL GUS EXPRESSION PATTERNS FOLLOWING TRANSPOSITION OF AN ENHANCER TRAP DS ELEMENT IN ARABIDOPSIS, MGG. Molecular & general genetics, 249(4), 1995, pp. 357-365
Citations number
43
Categorie Soggetti
Genetics & Heredity",Biology
ISSN journal
00268925
Volume
249
Issue
4
Year of publication
1995
Pages
357 - 365
Database
ISI
SICI code
0026-8925(1995)249:4<357:NGEPFT>2.0.ZU;2-F
Abstract
Enhancer trap derivatives of the maize Dissociation (Ds) transposon we re introduced into Arabidopsis thaliana. The enhancer trap Ds was so d esigned that upon transposition to sites containing regulatory sequenc es in adjacent genomic DNA, transcription of a Ds-borne beta-glucuroni dase (GUS) gene would be activated. Sixty percent of all transposition events were associated with GUS expression patterns including one lin ked to a mutant phenotype. Patterns of GUS expression were found in va rious organs and were stably inheritable in the F-4 and F-5 progenies. These results demonstrate the potential value of the technique as a m eans for detection of developmentally regulated genes and analysis of their function. The enhancer trap construct used in our experiments, a s well as the seeds of primary transformants are publicly available.