Objectives. The pulpal reaction and the marginal sealing of in vivo re
stored samples using resin composite and Scotchbond Multi-Purpose adhe
sive system (3M Dental Products) were analyzed in this study. Methods.
Twelve Class I non-exposed cavity preparations were placed on premola
rs to be removed for orthodontic reasons. They were restored and obser
ved at 7 d and 28 d. Results. The examination of the resin-dentin inte
rface under the scanning electron microscope (SEM) revealed: 1) a gap-
free attachment between adhesive resin and dentinal surface in 80% of
the areas studied, 2) penetration of resin tags into the dentinal tubu
les, and 3) formation of a 3-5 mu m thick acid-resistant hybrid layer.
Microfissures measuring about 10 mu m were observed in only 20% of th
e areas studied, these were located along the walls of the cavities, e
specially near the enamel in zones where there was a lower concentrati
on of dentinal tubules. The histological analysis, carried out 7 d aft
er preparation of the restoration, did not show any alteration of the
pulp. After 4 wk, reparative dentin was produced in the pulpal areas c
orresponding to the restored cavities. Significance. The quantity of n
ewly formed dentin is correlated with the distance from the cavity to
the pulp. The results indicate that acid-etching of vital dentin using
10% maleic acid does not impair pulpal healing in deep Class I caviti
es and that the Scotchbond Multi-Purpose adhesive system is able to pr
eserve the morphological and biological integrity of the pulpo-dentina
l complex.