CRYSTALLIZATION OF THE COMPLEX OF HUMAN IFN-GAMMA AND THE EXTRACELLULAR DOMAIN OF THE IFN-GAMMA RECEPTOR

A complex of human interferon-gamma (IFN-gamma) with the soluble extra cellular domain of the IFN-gamma receptor or-chain (IFN-gamma-R) has b een crystallised. Crystals of the complex were grown using PEG 4000 as the precipitating agent in the presence of beta-octyl glucoside. The receptor-ligand complex crystallizes in a monoclinic space group and d iffracts to about 3.0 Angstrom resolution, Isomorphous crystals have b een obtained with complex containing selenomethionine and cysteine mut ants of IFN-gamma, which may facilitate the ongoing X-ray structure de termination. (C) 1995 Wiley-Liss, Inc.