CRYSTALLIZATION AND X-RAY-ANALYSIS OF A SINGLE FAB BINDING DOMAIN FROM PROTEIN-L OF PEPTOSTREPTOCOCCUS-MAGNUS

Protein L is a multidomain cell wall constituent of certain strains of Peptostreptococcus magnus which binds to the variable domain of immun oglobulin kappa-light chains, A single immunoglobulin-binding domain o f M(r) = 9000 from this protein has been isolated and crystallized, Th e crystals are of space group P4(2)2(1)2, with cell dimensions a = b = 66.9 Angstrom, c = 68.3 Angstrom, and diffract to at least 2.2 Angstr om resolution, The asymmetric unit of the crystal contains two molecul es of the protein L domain, related by a noncrystallographic 2-fold ax is, as revealed by a self-rotation function calculated with native dif fraction data. (C) 1995 Wiley-Liss, Inc.