AVIAN MYCOPLASMA IDENTIFICATION USING POLYMERASE CHAIN-REACTION AMPLICON AND RESTRICTION-FRAGMENT-LENGTH-POLYMORPHISM ANALYSIS

A general mycoplasma polymerase chain reaction (PCR) was used to gener ate amplicon (DNA amplification product) from nine avian mycoplasma sp ecies. The PCR amplicons were reacted with 24 restriction enzymes, and the electrophoretic patterns of restriction fragment length polymorph ism (RFLP) were evaluated for differences between species of mycoplasm as. Four (DraI, MseI, RsaI, Tsp509I) of the 24 restriction enzymes cut the PCR amplicon of all nine mycoplasma species. The nine avian mycop lasma species could be distinctly differentiated using the RFLP analys is of the PCR amplicon.