DIFFERENT MECHANISMS OF INHIBITION OF DNA-SYNTHESIS BY (E)-5-(2-BROMOVINYL)-2'-DEOXYURIDINE IN CELLS TRANSFECTED WITH GENE FOR THYMIDINE KINASE OF HERPES-SIMPLEX VIRUS TYPE-1 AND IN CELLS INFECTED WITH THE VIRUS

The effect of (E)-5-(2-bromovinyl)-2'-deoxyuridine (BVDU) on deoxyribo nucleoside S-triphosphate pools was studied in cells transfected with gene for thymidine kinase of herpes simplex virus type 1 and cells inf ected with the virus. When infected cells were treated with BVDU, the triphosphate form of the nucleoside analog was detected. When transfec ted cells were treated with BVDU, the triphosphate form was not detect ed and the pattern of changes in the pools was the same as after 5-flu oro-2'-deoxyuridine treatment. BVDU seems to inhibit DNA synthesis dif ferently in the two cell lines and nucleotide metabolism in the transf ected cells was not the same as in the infected cells.