MOLECULAR MECHANISM OF 5-FLUORO-2'-DEOXYURIDINE-INDUCED DNTP IMBALANCE CELL-DEATH - PURIFICATION OF AN ENDONUCLEASE INVOLVED IN DNA DOUBLE-STRAND BREAKS DURING DNTP IMBALANCE DEATH

Authors
HWANG H OHTANI M NAKAZAWA T IGAKI T MASUI O KANKAWA S NAKAYAMA C YOSHIDA S YOSHIOKAHIRAMOTO A WATAYA Y
Citation
H. Hwang et al., MOLECULAR MECHANISM OF 5-FLUORO-2'-DEOXYURIDINE-INDUCED DNTP IMBALANCE CELL-DEATH - PURIFICATION OF AN ENDONUCLEASE INVOLVED IN DNA DOUBLE-STRAND BREAKS DURING DNTP IMBALANCE DEATH, Nucleosides & nucleotides, 14(9-10), 1995, pp. 2089-2097
Citations number
24
Categorie Soggetti
Biology
Journal title
Nucleosides & nucleotides → ACNP
ISSN journal
07328311
Volume
14
Issue
9-10
Year of publication
1995
Pages
2089 - 2097
Database
ISI
SICI code
0732-8311(1995)14:9-10<2089:MMO5DI>2.0.ZU;2-#
Abstract
We have detected, isolated and purified an endonuclease from 5-fluoro- 2'-deoxyuridine-treated FM3A cells. The molecular mass of the endonucl ease was approximately 40 kDa as judged by sodium dodecyl sulfate-poly acrylamide DNA-containing gel electrophoresis. The endonuclease causes double strand breaks in DNA, with an optimum pH at 6.