We present an index of centers of density for identifying areas of hig
h conservation value. This index represents the average importance of
an area to species occurring there as measured by comparisons of relat
ive densities of each species among avens. We evaluated the index usin
g collections of stream fishes from the Clinch River system above Norr
is Reservoir in Virginia and Tennessee (U.S.A.). A strong correlation
between index values measured at the same sites at different times sug
gested that the index could be applied to sites without replicated sam
ples in the region and still allow centers of density to be distinguis
hed from non-centers. Species richness showed no relationship to the i
ndex, suggesting that conservation priorities based solely on species
richness can be inadequate. A species-accumulation curve based on the
index performed nearly as well as one based on an algorithm for identi
fying the minimum number of sites in which all species in the region a
re represented, This pattern reflected the tendency of the index To we
ight regionally rare species more heavily than common species. But sit
es with high index values were not necessarily those selected by the a
lgorithm because the algorithm used only presence/absence, whereas the
index used the additional information present in relative densities.
Our index represents an additional tool for identifying ''hot spots''
of diversity, but conservation of biodiversity over the long term will
also require that the ecological integrity of regional landscape be m
aintained.