CLONING OF A DNA REPEAT ELEMENT FROM HORSE - DNA-SEQUENCE AND CHROMOSOMAL LOCALIZATION

A DNA repeat element, revealed initially by digestion of horse DNA wit h TaqI, was cloned and characterized by Southern and in situ hybridiza tion studies and nucleotide sequencing. The clone, e4/1, consisted of 32 tandem reiterations of a unit repeat of 21-22 bp, and produced mult ilocus DNA fingerprinting profiles that were useful for parentage anal ysis in horses. The tandem repeat element was shown by in situ hybridi zation to be localized in the centromeres of the acrocentric but not m etacentric classes of horse chromosomes.