MICROSATELLITE SEQUENCES IN PINUS-SYLVESTRIS

Scots pine (Pinus sylvestris) genomic libraries were constructed and s creened with oligonucleotide probes (GT)(10),(CT)(10), and (AT)(10). E ight microsatellites were identified from 6000 clones screened. The lo ngest microsatellite stretch found (GT)(9)(N)(21)(AT)(24), was amplifi ed from bud and single pollen grain samples. In order to clarify the c omplex amplification pattern revealed, two PCR products were sequenced . The size differences were caused both by varying repeat numbers of t he microsatellite stretches and by differences in other parts of the a mplified sequence. This kind of complex molecular basis of microsatell ite amplification within a species has not been previously reported. M icrosatellite sequences were used as PCR primers to detect polymorphis ms and to estimate the abundance of microsatellites.