Mhm. Sanago et al., MORPHOREGULATORY ROLE OF THIDIAZURON - MORPHOGENESIS OF ROOT OUTGROWTHS IN THIDIAZURON-TREATED GERANIUM (PELARGONIUM X HORTORUM BAILEY), Plant cell reports, 15(3-4), 1995, pp. 205-211
Root outgrowths formed on the root tissue of geranium (Pelargonium x h
ortorum Bailey cv. Kim and cv. Shone Helena) plants in response to tre
atment with the phenylurea derivative, thidiazuron (N-phenyl-N'-1,2,3-
thiadiazol-5'-ylurea; TDZ). Treatment with the cytokinin N-6-benzylami
nopurine (BAP) or the auxin alpha-naphthaleneacetic acid (NAA) did not
result in stimulation of similar abnormal structures on the root tiss
ue. Significantly more outgrowths developed on roots of plants treated
with 10 mu M and 20 mu M TDZ than on control plants or those treated
with 1 mu M TDZ for the eight-week treatment period. Some outgrowths p
roduced shoots and plantlets while still attached to roots, and regene
rants were easily separated from the root tissue and transferred to so
il in the greenhouse where they grew to maturity. Histological observa
tions suggested these outgrowths originated from the vascular cambium
region of the root.