MONITORING TRICHLOROETHYLENE MINERALIZATION BY PSEUDOMONAS-CEPACIA G4PR1

Citation
Pp. Luu et al., MONITORING TRICHLOROETHYLENE MINERALIZATION BY PSEUDOMONAS-CEPACIA G4PR1, Applied microbiology and biotechnology, 44(1-2), 1995, pp. 259-264
Citations number
17
Categorie Soggetti
Biothechnology & Applied Migrobiology
ISSN journal
01757598
Volume
44
Issue
1-2
Year of publication
1995
Pages
259 - 264
Database
ISI
SICI code
0175-7598(1995)44:1-2<259:MTMBPG>2.0.ZU;2-I
Abstract
To analyze the extent of mineralization of trichloroethylene (TCE) wit hout disturbing an actively growing biofilm, a minimal growth medium w as formulated that reduces the concentration of chloride ions to the e xtent that the chloride ions generated from TCE mineralization may be detected with a chloride-ion-specific electrode. By substituting chlor ide salts with phosphates and nitrates, a chloride-free minimal medium was produced that yields a specific growth rate for Pseudomonas cepac ia G4 PR1 which was 93% of that in chloride-ion-containing minimal med ium. Furthermore, TCE degradation by resting cell suspensions was simi lar in both media (85% of 75 mu M TCE degraded in 6 h), and complete m ineralization of TCE was slightly superior in the chloride-free minima l medium (77% compared to 60% of 75 mu M TCE mineralized in 6 h). In a ddition, indole-containing, minimal-medium agar plates were developed to indicate the presence of the TCE-degrading enzyme toluene ortho-mon ooxygenase (fire-engine-red colonies) as well as to distinguish this e nzyme from other TCE-degrading enzymes (toluene dioxygenase and toluen e para-mono-oxygenase).