IMMUNOCYTOCHEMICAL DEMONSTRATION OF PEROXISOMES IN CULTURED LEYDIG-CELLS - A DECREASE IN TESTOSTERONE SECRETION PRECEDES REDUCTION OF PEROXISOMES

Peroxisomes were visualized in primary cultures of mouse Leydig cells by light microscopic immunocytochemical demonstration of catalase and two enzymes of peroxisomal lipid beta-oxidation: acyl-CoA oxidase and 3-ketoacyl-CoA thiolase. Immunofluorescent peroxisomes had the form of granules, rods or short tubules located in the cytoplasm of Leydig ce lls. The amount of peroxisomes in the cells and the intensity of their fluorescence was similar irrespective of the antibody used. On days 2 and 4 of the culture, most Leydig cells contained very numerous, dens ely packed peroxisomes. On days 6 and 8, their number and fluorescence intensity gradually decreased. The parallel measurements of testoster one concentration in the culture medium revealed a sharp decline of an drogen production between days 1 and 4 of the culture. The observed pr ecedence of the decrease in testosterone production in relation to red uction of the peroxisome number suggests that a decrease in testostero ne level or factors regulating androgen synthesis may influence degrad ation of peroxisomes.