AN EFFICIENT SYNTHESIS OF SIALOGLYCOCONJUGATES ON A PEPTIDASE-SENSITIVE POLYMER SUPPORT

A novel method for the enzymatic synthesis of oligosaccharide derivati ves on a alpha-chymotrypsin-sensitive polymer support is described. Th e primer polymer having N-acetyl-D-glucosamine (GlcNAc) residue throug h a phenylalanine-containing spacer moiety was successfully elongated with galactosyl and sialyltransferases to give a glycopolymer bearing sialyl alpha(2 --> 6) N-acetyllactosamine branches in high yield. Subs equent hydrolysis with alpha-chymotrypsin proceeded smoothly and affor ded a versatile sialotrisaccharide derivative having a terminal amino group which can he used for creating neoglycoconjugates.