IMMOBILIZED-ENZYME ACTIVITY UNDER ISOTHERMAL AND NONISOTHERMAL CONDITIONS - STUDIES ON BETA-GALACTOSIDASE AS A MODEL ENZYME

Authors
MITA DG PORTACCIO M RUSSO P STELLATO S TOSCANO G BENCIVENGA U CANCIGLIA P DACUNTO A PAGLIUCA N ROSSI S GAETA FS
Citation
Dg. Mita et al., IMMOBILIZED-ENZYME ACTIVITY UNDER ISOTHERMAL AND NONISOTHERMAL CONDITIONS - STUDIES ON BETA-GALACTOSIDASE AS A MODEL ENZYME, Biotechnology and applied biochemistry, 22, 1995, pp. 281-294
Citations number
30
Categorie Soggetti
Biology,"Biothechnology & Applied Migrobiology
Journal title
Biotechnology and applied biochemistry → ACNP
ISSN journal
08854513
Volume
22
Year of publication
1995
Part
3
Pages
281 - 294
Database
ISI
SICI code
0885-4513(1995)22:<281:IAUIAN>2.0.ZU;2-5
Abstract
The behaviour of catalytic reactions was studied when an enzyme is imm obilized to a membrane interposed between two solutions of identical s ubstrate concentration, kept at different temperatures. beta-Galactosi dase was studied as a model enzyme. In the presence of a temperature g radient the apparent enzyme activity is higher than in comparable isot hermal conditions. The activity increase is proportional to the applie d transmembrane temperature difference. Two possible explanations are proposed for this behaviour. Various biotechnological applications are suggested.