UV-INDUCED PHOTOPRODUCTS IN HUMAN SKIN EXPLANTS ANALYZED BY TLC AND HPLC-RADIOACTIVITY DETECTION

Authors
BYKOV VJ HEMMINKI K
Citation
Vj. Bykov et K. Hemminki, UV-INDUCED PHOTOPRODUCTS IN HUMAN SKIN EXPLANTS ANALYZED BY TLC AND HPLC-RADIOACTIVITY DETECTION, Carcinogenesis, 16(12), 1995, pp. 3015-3019
Citations number
34
Categorie Soggetti
Oncology
Journal title
Carcinogenesis → ACNP
ISSN journal
01433334
Volume
16
Issue
12
Year of publication
1995
Pages
3015 - 3019
Database
ISI
SICI code
0143-3334(1995)16:12<3015:UPIHSE>2.0.ZU;2-Q
Abstract
The objective of this study was to apply the P-32-postlabelling method for the detection of UV-induced dipyrimidine photoadducts in human sk in explants. [P-32]TLC and [P-32]HPLC analysis were used. These techni ques allow detection of different combinations of cyclobutane dimers a nd 6-4 photoproducts in human skin at low doses of irradiation. Detect ion limit of the present method for some dinucleotide adducts is <1 ad duct per 10(7) normal nucleotides. Reaction of photochemical reversion of the cyclobutane dimers was performed to confirm the nature of the products detected in human skin explants.