ON THE MOBILITY OF IMMUNOGLOBULINES-G IN LANGMUIR-BLODGETT-FILMS

The reorganisation of Langmuir-Blodgett (LB) films of Immunoglobulines G deposited onto SnO2 substrates has been proved by fluorescence opti cal microscopy. By exploiting the quenching of the fluorescence signal due to the proximity of a fluorochrome to a conductive plane, it was possible to demonstrate that antibodies in LB films can move among the various deposited layers. This motion, which is prevented by fixing t he molecular layers by means of glutaraldehyde, appears to take place only during the transfer process of successive layers while building p rotein multilayer structures.