IDENTIFICATION OF IGE-BINDING EGG-WHITE PROTEINS - COMPARISON OF RESULTS OBTAINED BY DIFFERENT METHODS

The binding of IgE to egg white proteins was investigated for 34 sera from adults with a positive case history and/or positive RAST towards egg, and the impact of experimental conditions on IgE binding in commo nly used methods was studied. Radioimmunoblotting after SDS-PAGE of bo th reduced and unreduced egg white extracts showed complex reaction pa tterns. The results were confirmed by crossed radioimmunoelectrophores is (CRIE). Radio dot immunobinding was used to investigate the effect of treatment of allergens far SDS-PAGE and to evaluate the other metho ds. As a conclusion, the use of combinations of at least two methods i s recommended for the identification of IgE-binding egg white proteins . Of the 34 sera, 18 reacted with ovotransferrin, 13 with ovomucoid, 1 1 with ovalbumin and 5 with lysozyme. The amounts of IgE bound to oval bumin and lysozyme were generally lower than the amounts bound to ovot ransferrin and ovomucoid.