PURIFICATION OF CATIONIC PEROXIDASES BOUND IONICALLY TO THE CELL-WALLS FROM THE ROOTS OF ZINNIA-ELEGANS

Cell wall-bound and tracheary element-specific peroxidase isoenzymes, designated P5A and P5B, were shown previously to be associated with li gnification during the differentiation into tracheary elements of sing le cells isolated from the mesophyll of Zinnia elegans (Sate et al. Pl anta 189: 584-589, 1993; Planta 196: 141-147, 1995). Isoenzymes corres ponding to P5 (RP5A and RP5B) were present at a relatively high level in the roots of Zinnia elegans. These isoenzymes were purified from th e Zinnia roots by several column-chromatographic steps. Both RP5A and RP5B had molecular masses of 35 kDa. purified RP5A and RP5B were cleav ed by CNBr and the partial amino acid sequences of these isoenzymes we re determined.