Mk. Oaks et al., POLYMERASE CHAIN-REACTION CLONING AND EXPRESSION OF THE RAT GRANULOCYTE-MACROPHAGE COLONY-STIMULATING FACTOR, Journal of interferon & cytokine research, 15(12), 1995, pp. 1095-1102
We used reverse transcription-polymerase chain reaction (RT-PCR) to cl
one a rat complementary DNA that encoded the PVC rat granulocyte-macro
phage colony-stimulating factor (GM-CSF). PCR products were cloned int
o a eukaryotic expression vector and transfected into the mouse myelom
a cell line Sp2/0-Ag14. Cell culture supernatants of two of these tran
sfectants supported proliferation of the growth factor-dependent cell
line, DA-3, and promoted myeloid colony formation in rat and mouse bon
e marrow cell (BMC) cultures. The GM-CSF activity in these supernatant
s was neutralized by a polyclonal antibody to mouse GM-CSF, The clonin
g and expression of rat GM-CSF provides a valuable reagent for the stu
dy of the biology and clinical applications of the GM-CSFs.