THE EFFECTS OF CHRONIC AND ACUTE EXPOSURE TO HYDROGEN-SULFIDE ON ATLANTIC SALMON (SALMO-SALAR L)

In separate experiments, Atlantic salmon smelts (Salmo salar L.) were exposed to two different regimes of hydrogen sulphide. In the first ex periment, fish were exposed to chronic, periodic but sublethal levels of hydrogen sulphide (maximally 7.8 mu M) over a period of 18 weeks. R egular gill and liver samples were taken and growth determined relativ e to a control group. The difference in growth rate between treated an d control fish was small. Gill damage (clubbing, thickening of the sec ondary lamellae) peaked between 6 and 8 weeks after initial exposure. Subsequently there was evidence of an adaptive response such that afte r 16 weeks, gill tissues from exposed fish appeared healthy and normal . Liver damage was progressive, By Week 10 more than 50% of livers exa mined showed diffuse hepatic necrosis and diffuse vacuolar degeneratio n which increased to 80% by Week 18. In the second experiment, salmon were exposed to one, single, acute but sub-lethal dose (22 mu M or 29 mu M) of hydrogen sulphide over 20 min. The effect on gills, livers an d lactate dehydrogenase (LDH) activity was monitored over 14 days. For both concentrations, three salmon were sampled immediately, 1, 2; 3, 4, 6, 8, 10, 12 and 14 days after treatment. Gill tissues showed fusio n of the secondary lamellae and thickening of the primary lamellae. Li ver damage became evident 3 days after exposure. LDH activity was elev ated compared to control for 4 days after exposure.