LIPO-OLIGOSACCHARIDE OF CAMPYLOBACTER-LARI STRAIN PC-637 - STRUCTURE OF THE LIBERATED OLIGOSACCHARIDE AND AN ASSOCIATED EXTRACELLULAR POLYSACCHARIDE

Lipo-oligosaccharide from phenol-water extraction of cells of Campylob acter lari strain PC 637 was separated as a water-insoluble gel of low relative molecular mass (M(r)) from a water-soluble extracellular pol ysaccharide of high M(r). Structural investigations were performed on the lipo-oligosaccharide and the extracellular polysaccharide, various ly using H-1, C-13, and P-31 NMR spectroscopy, linkage analysis, and f ast atom bombardment-mass spectrometry of permethylated derivatives of the glycans and their products of chemical and enzymic degradation. T he following structures are proposed for the highly branched oligosacc haride region: GRAPHICS and for the tetraglycosyl phosphate repeatin g unit of the extracellular polysaccharide: GRAPHICS GRAPHICS