SCREENING OF YAC CLONES AND BUILDING A MAP OF THE CHROMOSOME-13 REGION OFTEN DELETED DURING CHRONIC B-CELL LYMPHOCYTIC LEUKOSIS

Pools of YAC clones from the ICRF library were analyzed by PCR using P BKpt, MGG15, and D13S25 markers that flank the chromosome 13 region of ten deleted during chronic lymphocytic leukemia. Ten clones were found and described. Nine mega-YAC clones from the CEPH library flanking th e region of interest were analyzed by PCR, which allowed building a ma p from 18 overlapping YAC clones and establishing the mutual arrangeme nt of 10 tested STS markers within the 13q14.3 region (seven of those being polymorphic microsatellite sites). The region where CLL-specific rearrangements occur is not longer than 1400 bp. The centromere-proxi mal end of the map overlaps with the published map that includes RBI g ene (retinoblastoma), while the telomere-proximal end overlaps with th e described one around the WD gene (Wilson-Konovalov disease). The tot al length of the united map of chromosome 13 segment is about 8 millio n bp.