TRANSIENT EXPRESSION OF HEPATITIS-VIRUS SURFACE-ANTIGEN (HBSAG) GENE IN MONKEY CELLS BY A SV40-BASED VIRUS VECTOR

Authors
JORGE SAC DELAHERA MC SPINA AMM PINHO JRR MENCK CFM
Citation
Sac. Jorge et al., TRANSIENT EXPRESSION OF HEPATITIS-VIRUS SURFACE-ANTIGEN (HBSAG) GENE IN MONKEY CELLS BY A SV40-BASED VIRUS VECTOR, Biotechnology letters, 17(12), 1995, pp. 1285-1290
Citations number
19
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Biotechnology letters → ACNP
ISSN journal
01415492
Volume
17
Issue
12
Year of publication
1995
Pages
1285 - 1290
Database
ISI
SICI code
0141-5492(1995)17:12<1285:TEOHS(>2.0.ZU;2-R
Abstract
We have constructed a recombinant SV40-based vector carrying the S gen e coding for the hepatitis B virus surface antigen (HBsAg). This vecto r replicates as an episome in monkey COS7 cells, producing high levels of hepatitis B virus surface antigen (HBsAg), which is liberated in t he cell medium, probably as a membrane vesicule. The vector also carri es the SV40-late genes and produces recombinant viruses. These viruses were used to infect fresh cell culture, with detection of HBsAg in th e medium. Thus, this virus vector can efficiently transduce the gene f or HBsAg.