ANAEROBIC BIOTRANSFORMATION OF 2,4-DINITROTOLUENE WITH ETHANOL AS PRIMARY SUBSTRATE - MUTUAL EFFECT OF THE SUBSTRATES ON THEIR BIOTRANSFORMATION

The effect of the initial concentration of 2,4-dinitrotoluene (DNT) on its biotransformation and on the microbial utilization of ethanol was investigated. The culture used in this study was acclimated in a cont inuous flow laboratory fermenter with 2,4-DNT and ethanol as substrate s under strict anaerobic conditions at 35 degrees C. Biochemical Metha ne Potential (BMP) tests were conducted in an anaerobic respirometer, and the acclimated culture from the fermenter served as the inoculum. 2,4-DNT was completely transformed to 2,4-diaminotoluene (DAT) under a naerobic conditions. Two intermediates, 2-amino-4-nitrotoluene (2-A-4- NT) and 4-amino-2-nitrotoluene (4-A-2-NT), were found during the biotr ansformation. 2,4-DNT inhibited its own biotransformation as well as t he acetogenesis of ethanol. The extent of the inhibition increased wit h the increase of the initial concentration of 2,4-DNT. Propionate was formed during the fermentation of ethanol without 2,4-DNT or with low initial concentrations of 2,4-DNT. Higher initial concentrations of 2 ,4-DNT caused a decrease in the extent of formation of propionate with acetate production being favored.