SCREENING AND CHARACTERIZATION OF GLUTARYL-7-AMINOCEPHALOSPORANIC ACID ACYLASE FROM PSEUDOMONAS SP

Authors
CHEN MC CHEN LL LEE YH WEN CY CHU WS
Citation
Mc. Chen et al., SCREENING AND CHARACTERIZATION OF GLUTARYL-7-AMINOCEPHALOSPORANIC ACID ACYLASE FROM PSEUDOMONAS SP, Biotechnology techniques, 9(12), 1995, pp. 859-862
Citations number
10
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Biotechnology techniques → ACNP
ISSN journal
0951208X
Volume
9
Issue
12
Year of publication
1995
Pages
859 - 862
Database
ISI
SICI code
0951-208X(1995)9:12<859:SACOGA>2.0.ZU;2-V
Abstract
Three screening methods were used to isolate GL-7-ACA acylase-producin g strains. Three positive isolates were identified with Pseudomonas ni troreducens CCRC 11041 possessing the highest activity, against GL-7-A CA and GL-7-ADCA. No activity was detected when Ceph C or succinyl-7-A CA was used as substrate; glutaric acid was found to be inhibitory. CC RC 11041 could produce maximal GL-7-ACA acylase activity when cultivat ed on meat extract medium Il. The enzyme had a pH optimum of 5.0 and a temperature optimum of 42 degrees C.