SIMPLE AND RAPID-DETERMINATION OF THE ACTIVITY OF RECOMBINANT D-AMINO-ACID OXIDASE IN CEPHALOSPORIN-C BIOCONVERSION WITH USE OF A MICRO PO(2) PROBE

Authors
KIM IW KHANG YH
Citation
Iw. Kim et Yh. Khang, SIMPLE AND RAPID-DETERMINATION OF THE ACTIVITY OF RECOMBINANT D-AMINO-ACID OXIDASE IN CEPHALOSPORIN-C BIOCONVERSION WITH USE OF A MICRO PO(2) PROBE, Biotechnology techniques, 9(12), 1995, pp. 863-868
Citations number
12
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Biotechnology techniques → ACNP
ISSN journal
0951208X
Volume
9
Issue
12
Year of publication
1995
Pages
863 - 868
Database
ISI
SICI code
0951-208X(1995)9:12<863:SAROTA>2.0.ZU;2-6
Abstract
D-Amino acid oxidase (DAAO) genes were amplified from Trigonopsis vari abilis and. Rhodotorula gracilis by polymerase chain reactions. T. var iabilis DAAO gene was cloned into a pUC19 vector. The expression of th e T. variabilis DAAO was directly determined in permeabilized E. coli cells using a micro pO(2) probe. The micro pO(2) probe was sensitive e nough to be suitable for a simple and rapid estimation of DAAO activit y toward cephalosporin. C.