RAPID CONSTRUCTION OF COMPETITIVE TEMPLATES FOR QUANTITATIVE PCR OF REVERSE-TRANSCRIBED MESSENGER-RNAS

Authors
DELGIACCO L DONEDA L GRAMMATICO P LARIZZA C
Citation
L. Delgiacco et al., RAPID CONSTRUCTION OF COMPETITIVE TEMPLATES FOR QUANTITATIVE PCR OF REVERSE-TRANSCRIBED MESSENGER-RNAS, Biotechnology techniques, 9(12), 1995, pp. 879-884
Citations number
1
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Biotechnology techniques → ACNP
ISSN journal
0951208X
Volume
9
Issue
12
Year of publication
1995
Pages
879 - 884
Database
ISI
SICI code
0951-208X(1995)9:12<879:RCOCTF>2.0.ZU;2-G
Abstract
A novel approach of competitive PCR for quantifying mRNAs is described where competitors are constructed by deleting an internal sequence fr om target reverse transcribed mRNAs. The two sets of product generated (control,and target) are easily discriminated by size and quantified for the sequence of interest and a housekeeping gene, beta-actin used as internal mRNA control.