IMPROVED DIFFERENTIATION OF BETA-GAL(-GAL PLATES CONTAINING MYCOLOGICAL PEPTONE() COLONIES ON X)

Authors
OKENNEDY R PATCHING JW
Citation
R. Okennedy et Jw. Patching, IMPROVED DIFFERENTIATION OF BETA-GAL(-GAL PLATES CONTAINING MYCOLOGICAL PEPTONE() COLONIES ON X), Biotechnology techniques, 9(12), 1995, pp. 921-924
Citations number
6
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Biotechnology techniques → ACNP
ISSN journal
0951208X
Volume
9
Issue
12
Year of publication
1995
Pages
921 - 924
Database
ISI
SICI code
0951-208X(1995)9:12<921:IDOBPC>2.0.ZU;2-N
Abstract
Inclusion of mycological peptone in the base medium of X-gal plates fa cilitates the detection of isolates of Saccharomyces cerevisiae produc ing beta-galactosidase due to improved colour development. Substitutio n of tryptone with mycological peptone in a standard Escherichia coli medium (LB) also facilitates colour development on X-gal plates and ob viates the need for an inducer. The addition of mycological peptone to the base medium of X-gal plates offers a cost-effective method to imp rove the detection of the beta-galactosidase gene in recombinant strai ns of S. cerevisiae and E. coli.