IN-VITRO PLANT-REGENERATION OF SCENTED-LEAVED GERANIUM PELARGONIUM GRAVEOLENS

Plants were regenerated in culture from leaf segments of the scented-l eaved geranium Pelargonium graveolens. Callus was induced on Murashige and Skoog (MS) medium supplemented with 5.0 mu M each of naphthalene acetic acid, 2,4-dichlorophenoxy-acetic acid and 2.5 mu M Of kinetin. Primary callus on transfer to MS medium supplemented with 20 mu M 2-is opentenyl adenine and 10 mu M kinetin induced maximum shoot formation. Addition of adenine sulfate at 108 mu M promoted prolific multiple sh oot formation. Excised plantlets were rooted in liquid medium in the p resence of indole-3-butyric acid or phloroglucinol. Regenerated plants were successfully established in soil.