A HYPERVARIABLE MIDDLE REPETITIVE DNA-SEQUENCE FROM CITRUS

The use of hypervariable sequences for DNA typing of plants is focusse d on microsatellites and on amplification of regions defined by random (RAPD) or defined (AFLP) primers for PCR analysis of genomes. A hyper variable length of middle repetitive DNA has been isolated from citrus that contains no obvious hypervariable structures. The fingerprinting probe was shown to have an important commercial application in the se paration of zygotic from nucellar progeny. A somatic variant of the se quence within one orange tree suggests that somatic variation in hyper variable markers may be a common event.