Schizophyllan, an extracellular polysaccharide secreted by the fungus
Schizophyllum commune ATCC 38548, shows interesting technical and phar
maceutical applications. Especially in the degraded form, this homoglu
can is applicable as an antitumor, antihepatitis, anti-HIV and antivir
al agent. Due to the fact that the side chains of the biopolymer are e
ssential for its activities, a regioselective degradation of the beta-
1,3 basic chain by maintaining the beta-1,6 side chains is necessary.
This paper covers investigations which deal with the regioselective cl
eavage of the basic chain. It was found that the hydrolysis of aqueous
polysaccharide solutions by incubation in DURAN glass bottles al 121
degrees C and 1 bar was the most successful method. A slight decrease
of pH, a rapid loss of viscosity, a constant increase in reducing end
groups and a continual release of glucose over an incubation time of 1
00 h indicated a degradation especially at the basic chain. Stepwise u
ltrafiltration of degraded solutions yielded fractions with varying mo
lar masses and the ratio of the fractions depended on the total incuba
tion time. The maintenance of the side chains was verified by C-13-NMR
spectra. The regioselectivity of this degradation method can explaine
d by a pore theory. One attempt to justify this theory is the suppress
ion of hydrolysis after hydrophobization of the glass surface by using
dichloromethylsilane.