Ge. Hannigan et al., REGULATION OF CELL-ADHESION AND ANCHORAGE-DEPENDENT GROWTH BY A NEW BETA(1)-INTEGRIN-LINKED PROTEIN-KINASE, Nature, 379(6560), 1996, pp. 91-96
THE interaction of cells with the extracellular matrix regulates cell
shape, motility, growth, survival, differentiation and gene expression
, through integrin-mediated signal transduction(1-3). We used a two-hy
brid screen to isolate genes encoding proteins that interact with the
beta(1)-integrin cytoplasmic domain. The most frequently isolated comp
lementary DNA encoded a new, 59K serine/threonine protein kinase, cont
aining four ankyrin-like repeats. We report here that this integrin-li
nked kinase (ILK) phosphorylated a beta(1)-integrin cytoplasmic domain
peptide in vitro and coimmunoprecipitated with beta(1) in lysates of
mammalian cells. Endogenous ILK kinase activity was reduced in respons
e to fibronectin. Overexpression of p59(ILK) disrupted epithelial cell
architecture and inhibited adhesion to integrin substrates, while ind
ucing anchorage-independent growth. We propose that ILK is a receptor-
proximal protein kinase regulating integrin-mediated signal transducti
on.