IDENTIFICATION OF A 25-KILODALTON PROTEIN OF MYCOBACTERIUM-BOVIS BCG TO DISTINGUISH BCG STRAINS FROM MYCOBACTERIUM-TUBERCULOSIS

Authors
KUMAR D SRIVASTAVA BS SINGH NB SRIVASTAVA R
Citation
D. Kumar et al., IDENTIFICATION OF A 25-KILODALTON PROTEIN OF MYCOBACTERIUM-BOVIS BCG TO DISTINGUISH BCG STRAINS FROM MYCOBACTERIUM-TUBERCULOSIS, Journal of clinical microbiology, 34(1), 1996, pp. 224-226
Citations number
15
Categorie Soggetti
Microbiology
Journal title
Journal of clinical microbiology → ACNP
ISSN journal
00951137
Volume
34
Issue
1
Year of publication
1996
Pages
224 - 226
Database
ISI
SICI code
0095-1137(1996)34:1<224:IOA2PO>2.0.ZU;2-X
Abstract
Mycobacterium bovis BCG vaccine strains were compared with A Mycobacte rium tuberculosis by sodium dodecyl sulfate-polyacrylamide gel electro phoresis, A 25-kDa protein observed in the BCG strains was absent in M . tuberculosis. Rabbit antibodies specific to the 25-kDa protein uniqu ely identified this protein in BCG strains but not in M. tuberculosis. It is suggested that the 25-kDa protein acid polyclonal antibodies di rected against this antigen can be exploited to distinguish BCG strain s from M. tuberculosis.