2,8-DIAZIDO-ATP - A SHORT-LENGTH BIFUNCTIONAL PHOTOAFFINITY LABEL FORPHOTOAFFINITY CROSS-LINKING OF A STABLE F1 IN ATP SYNTHASE (FROM THERMOPHILIC BACTERIA PS3)

Authors
SCHAFER HJ RATHGEBER G KAGAWA Y
Citation
Hj. Schafer et al., 2,8-DIAZIDO-ATP - A SHORT-LENGTH BIFUNCTIONAL PHOTOAFFINITY LABEL FORPHOTOAFFINITY CROSS-LINKING OF A STABLE F1 IN ATP SYNTHASE (FROM THERMOPHILIC BACTERIA PS3), FEBS letters, 377(3), 1995, pp. 408-412
Citations number
37
Categorie Soggetti
Biophysics,Biology
Journal title
FEBS letters → ACNP
ISSN journal
00145793
Volume
377
Issue
3
Year of publication
1995
Pages
408 - 412
Database
ISI
SICI code
0014-5793(1995)377:3<408:2-ASBP>2.0.ZU;2-6
Abstract
To demonstrate the direct interfacial position of nucleotide binding s ites between subunits of proteins me hate synthesized the bifunctional photoaffinity label 2,8-diazidoadenosine 5'-triphosphate (2,8-DiN(3)A TP). UV irradiation of the F-1-ATPase (TF1) from the thermophilic bact erium PS3 in the presence of 2,8-DiN(3)ATP results in a nucleotide-dep endent inactivation of the enzyme and in a nucleotide-dependent format ion of alpha-beta cross-links. The results confirm an interfacial loca lization of all the nucleotide binding sites on TF1.