Ev. Korolev et al., TUBULAR STRUCTURES OF MYCOPLASMA-GALLISEPTICUM AND THEIR POSSIBLE PARTICIPATION IN CELL MOTILITY, Microbiology, 140, 1994, pp. 671-681
In five strains of Mycoplasma gallisepticum, a protein with a molecula
r mass of about 40 kDa was detected by immunoblotting with anti-pig br
ain tubulin polyclonal and monoclonal antibodies. in eight other mycop
lasma species similarly tested no reaction was observed. Thin serial s
ections of M. gallisepticum and Acholeplasma laidlawii cells examined
by transmission electron microscopy revealed a submembrane system of t
ubules in M. gallisepticum but not in A. laidlawii. The intracellular
spatial distribution of the tubular structures was reconstructed. Thin
sections of M. gallisepticum treated with anti-tubulin antibodies and
colloidal gold particles (immunogold labelling) revealed distinct lab
elling of the tubular system. Analysis of the tubular structures by hi
gh resolution electron microscopy and optical diffraction showed their
helical organization to be: diameter 40 nm, helix pitch approximately
20 nm and electron-transparent core 10 nm in diameter. A possible inv
olvement of the tubular system in mycoplasma motility is suggested.