HUMAN PAPILLOMAVIRUS-11 E5A DELAYS THE GROWTH RESTRICTION INDUCED BY TEMPERATURE SHIFT IN TEMPERATURE-SENSITIVE SIMIAN-VIRUS-40-T ANTIGEN-IMMORTALIZED KERATINOCYTES

The transfection with human papillomavirus type II E5a oncoprotein can cause longer and more active proliferation in the human keratinocytes previously transfected stably with temperature sensitive SV40 T antig en at 39 degrees C, Also, after the E5a transfection in parental tempe rature sensitive SV40 T antigen gene transfected cells, we observe a d elay in the accumulation of p21 gene at 39 degrees C. Moreover, T anti gen degradation did not occur at 39 degrees C in the E5a transfected c ells as it did in the parental cells. We draw from these observations that E5a may transiently stabilize temperature sensitiveT antigen and/ or repress p21 synthesis. (C) 1995 Academic Press, Inc.