ESTABLISHMENT OF IMMORTAL HEPATOCYTES FROM A CYP3A7-TRANSGENIC P53-KNOCKOUT MOUSE

Authors
SHIMOJI M HATTORI K ITOH S NAKAYAMA K KATSUKI M AIZAWA S YOKOI T KAMATAKI T
Citation
M. Shimoji et al., ESTABLISHMENT OF IMMORTAL HEPATOCYTES FROM A CYP3A7-TRANSGENIC P53-KNOCKOUT MOUSE, Biochemical and biophysical research communications, 217(3), 1995, pp. 1001-1005
Citations number
10
Categorie Soggetti
Biology,Biophysics
Journal title
Biochemical and biophysical research communications → ACNP
ISSN journal
0006291X
Volume
217
Issue
3
Year of publication
1995
Pages
1001 - 1005
Database
ISI
SICI code
0006-291X(1995)217:3<1001:EOIHFA>2.0.ZU;2-W
Abstract
Immortal hepatocytes were established from a CYP3A7-transgenic/p53-kno ckout mouse. The cells could be maintained with shorter doubling time after culture for 1 month. Detectable amounts of CYP3A7 mRNA and the a ctivity of 7-propoxycoumarin O-depropylase, one of the representative CYP3A activities, were present in the immortal hepatocytes. Albumin mR NA, which is specifically expressed in the liver, remained in these ce lls. (C) 1995 academic Press, Inc.