OBJECTIVE: Our purpose was to compare the protein isoform composition
of the contractile apparatus at different levels of the fetoplacental
vessel musculature at term. STUDY DESIGN: Umbilical, chorionic, and st
em villi vessel protein extracts were run on one- and two-dimensional
gel electrophoresis; previously characterized human myometrium protein
s were used as the smooth muscle proteins of reference. RESULTS: Fetop
lacental vessel musculature exhibited a high actin/myosin ratio. The p
resence, in varying quantities, of myosin heavy chain and actin isofor
ms of smooth muscle type in the different vessels reflected their degr
ee of differentiation. The presence of nonmuscle protein isoforms, par
ticularly in stem villi vessels, indicated a certain degree of immatur
ity. CONCLUSIONS: The presence of smooth muscle contractile protein is
oforms indicates that fetoplacental vessel musculature is highly diffe
rentiated. Regional modulation of fetoplacental blood flow could be, i
n part, the result of local differences in contractile apparatus prote
in composition.