A COMPARISON OF SOME LEUKOCYTE DIFFERENTIATION MARKERS AND THE ADENOSINE-DEAMINASE AND PURINE NUCLEOSIDE PHOSPHORYLASE VALUES IN B-CELL ANDT-CELL LEUKEMIAS AND LYMPHOMAS

Peripheral blood, bone marrow and/or lymph nodes of 77 patients with T - and B-ALLs/lymphomas were characterized for their surface membrane m arker profiles using flow cytometry and fluorescence microscopy. Purin e metabolism enzyme activities were compared with membrane immunopheno types. T and B-ALLs/lymphomas subtypes were defined by the expression of surface membrane antigens detected by the monoclonal antibodies. Ba sed on immunophenotyping we found the following characteristic marker profiles: in T-ALL - CD7, CD2, CD1, CD5, CD3, CD4, CD8, CD38, CD71; in T-NHL - CD7, CD2, CD3, CD4, CD5, CD6; in pre-B ALL - CD10, CD19, CD24 , HLA-DR, CD34, in B-ALL - CD19, CD20, CD24, HLA-DR, SmIg with kappa o r lambda light chains; in B-CLL - weak SmIg, kappa or lambda, CD19, CD 20, CD24, CD5, HLA-DR; in B-NHL - CD19, CD20, CD22, CD24, CD5, more in tensive SmIg, kappa or lambda. The cells of leukemic cases tended to h ave more immature phenotypes than those of lymphoma cases. Analysis of purine metabolism enzyme activities showed that there was a correlati on between the values of adenosine deaminase (ADA) and purine nucleosi de phosphorylase (PNP) and various types of T- and B-ALLs/lymphomas. A DA levels in B-NHL and B-CLL were lower than those in normal cells, wh ile ADA level in T-ALL, T-NHL, pre-B-ALL and B-ALL was higher (the ave rage 185, 92, 73, 63 pkat.10(-6) cells, respectively).ADA activity was significantly different between lymphocytes of control group and T-AL L (p < 0.01), between T-ALL and T-NHL (p < 0.05), between T-NHL and B- NHL (p < 0.05) and between T-ALL and B-NHL (p < 0.05). PNP activities were lower to those in normal cells. ADA/PNP ratio increased mostly in T-ALL, less in T-NHL, pre-B-ALL and B-ALL (10.8 and 5.3 and 2.2 and 2 .0, respectively). ADA/PNP ratio was significantly different between T -ALL and pre-B-ALL (p < 0.05) and between T-ALL and B-NHL (p < 0.05).