ANALYSIS OF THE PHENOTYPE AND CELLULAR DNA CONTENT IN SOME LEUKEMIAS BY FLOW-CYTOMETRY

Phenotype and cell cycle distribution in peripheral blood and bone mar row mononucleated cells was studied in patients with different leukemi as: T-ALL, AML, CLL, CML and plasmocytoma. DNA flow cytometry with pro pidium iodide fluorescence was used. Differences in cell cycle between mononucleated cells from T-ALL and CML patients on one hand and norma l controls on the other were seen in peripheral blood but not in bone marrow specimens. Patients with AML, CLL and plasmocytoma showed a cel l cycle distribution of mononucleated cells similar to normal controls . DNA content analysis in some leukemias was discussed.