CONDITIONS FOR OPTIMAL PRODUCTION OF ACTI VE OXYGEN FORMS BY POLYMORPHONUCLEAR RAT LEUKOCYTES

Using the procedure of lysis by ammonium chloride followed by differen tiation centrifuging, the fraction of cells containing polymorphonucle ar leucocytes was isolated from the whole rat blood to stady the condi tions of optimal production of oxygen forms was determined from the ch emiluminescent cell response in the presence of luminol. It was shown that chemiluminescent cell response depends on the temperature, pH and osmolarity of the medium as well as the on the presence of various co ncentrations of Ca2+ in the incubation medium. The maximal chemilumine scent response of polymorphonuclear leucocytes was observed at 35-37 d egrees C, pH 7,6, osmolarity of 0,15 and Ca2+ concentration of 5-10 mM .