M. Hisamatsu et al., EFFECTS OF DEGRADED SCHIZOPHYLLANS ON REGENERATION OF PROTOPLAST CELLS OF SACCHAROMYCES-CEREVISIAE, Bioscience, biotechnology, and biochemistry, 59(12), 1995, pp. 2307-2308
Schizophyllan was heated at 100 degrees C in 85% dimethyl sulfoxide (D
MSO) containing 0.01 M H2SO4 for various times, and fractionated by ge
l-permeation chromatography. Molecular weights (M(r)) of the depolymer
ized products thus obtained were measured in water and DMSO by GPC-LAL
LS to estimate their conformations in water. The products with triple
helical structure stimulated regeneration of Saccharomyces cerevisiae
protoplast cells, while those of single chain conformation were totall
y inactive.