SURFACE LOCALIZATION, REGULATION, AND BIOLOGIC PROPERTIES OF THE 96-KDA ALCOHOL ALDEHYDE DEHYDROGENASE (EHADH2) OF PATHOGENIC ENTAMOEBA-HISTOLYTICA

Authors
FLORES BM STANLEY SL YONG TS ALI M YANG WG DIEDRICH DL TORIAN BE
Citation
Bm. Flores et al., SURFACE LOCALIZATION, REGULATION, AND BIOLOGIC PROPERTIES OF THE 96-KDA ALCOHOL ALDEHYDE DEHYDROGENASE (EHADH2) OF PATHOGENIC ENTAMOEBA-HISTOLYTICA, The Journal of infectious diseases, 173(1), 1996, pp. 226-231
Citations number
24
Categorie Soggetti
Infectious Diseases
Journal title
The Journal of infectious diseases → ACNP
ISSN journal
00221899
Volume
173
Issue
1
Year of publication
1996
Pages
226 - 231
Database
ISI
SICI code
0022-1899(1996)173:1<226:SLRABP>2.0.ZU;2-C
Abstract
The 96-kDa surface antigen of Entamoeba histolytica was demonstrated t hrough extensive immunologic evaluation with monoclonal and monospecif ic antibodies to be identical to or an isoform of the amebic alcohol/a ldehyde dehydrogenase (EhADH2), EhADH2 was secreted, excreted, or shed into the culture medium in quantities commensurate with amebic growth when studied in a novel culture system. Of importance, using RNase pr otection assays, specific mRNA coding for the EhADH2 gene product(s) w as up-regulated by treatment of viable trophozoites with the enzyme su bstrate ethanol, These data provide insight into the biology of this e nzyme and its regulation by appropriate stressors.